Awakening dormant glycosyltransferases in CHO cells with CRISPRa.
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ABSTRACT:
SUBMITTER: Karottki KJC
PROVIDER: S-EPMC7261405 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Publications
Awakening dormant glycosyltransferases in CHO cells with CRISPRa.
Karottki Karen Julie la Cour KJC Hefzi Hooman H Xiong Kai K Shamie Isaac I Hansen Anders Holmgaard AH Li Songyuan S Pedersen Lasse Ebdrup LE Li Shangzhong S Lee Jae Seong JS Lee Gyun Min GM Kildegaard Helene Faustrup HF Lewis Nathan E NE
Biotechnology and bioengineering 20191112 2
Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes. We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were ...[more]
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