Unknown

Dataset Information

0

Circular RNA hsa_circ_0131242 Promotes Triple-Negative Breast Cancer Progression by Sponging hsa-miR-2682.


ABSTRACT: Objective:CircRNAs are emerging as vital regulators in a variety of cancers. However, the expression pattern and potential mechanism of circRNAs in triple-negative breast cancer remain unclear. In this study, we aim to systematically investigate circRNAs alteration in triple-negative breast cancer tissues. Methods:Microarray and bioinformatics analyses were used to identify circRNAs expression in cancer tissues. qRT-PCR was conducted to measure the expression of RNAs. Cell Counting Kit-8, wound-healing and transwell assays were conducted to investigate the function of circRNAs. Dual-luciferase reporter assay was performed to validate target binding. Results:Hsa_circ_0131242 was highly expressed in both cancer tissues and cell lines compared to control. Subsequently, statistical analyses revealed that high expression of hsa_circ_0131242 was positively correlated with advanced tumor stages and poorer clinical features in cancer patients. Hsa_circ_0131242 knockdown could suppress the progression of breast cancer cells. Bioinformatics prediction and luciferase reporter assay showed that hsa_circ_0131242 acted as a sponge for hsa-miR-2682. Moreover, co-transfection of hsa-miR-2682 inhibitor and si-hsa_circ_0131242 rescued cell proliferation and migration in BT549 and MDA-MB-468 cell lines. Conclusion:Our study identified hsa_circ_0131242 expression in TNBC for the first time and found that hsa_circ_0131242 may promote triple-negative breast cancer progression by sponging hsa-miR-2682.

SUBMITTER: Li Y 

PROVIDER: S-EPMC7261810 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Circular RNA hsa_circ_0131242 Promotes Triple-Negative Breast Cancer Progression by Sponging hsa-miR-2682.

Li Yueting Y   Shi Pengxu P   Zheng Tianzhi T   Ying Ziwei Z   Jiang Daqing D  

OncoTargets and therapy 20200527


<h4>Objective</h4>CircRNAs are emerging as vital regulators in a variety of cancers. However, the expression pattern and potential mechanism of circRNAs in triple-negative breast cancer remain unclear. In this study, we aim to systematically investigate circRNAs alteration in triple-negative breast cancer tissues.<h4>Methods</h4>Microarray and bioinformatics analyses were used to identify circRNAs expression in cancer tissues. qRT-PCR was conducted to measure the expression of RNAs. Cell Countin  ...[more]

Similar Datasets

| S-EPMC10731075 | biostudies-literature
| S-EPMC8331001 | biostudies-literature
| S-EPMC7653573 | biostudies-literature
| S-EPMC6933405 | biostudies-literature
| S-EPMC6343010 | biostudies-literature
| S-EPMC6657235 | biostudies-literature
| S-EPMC8564996 | biostudies-literature
| S-EPMC6675706 | biostudies-literature
| S-EPMC7249963 | biostudies-literature
| S-EPMC9688127 | biostudies-literature