Ontology highlight
ABSTRACT:
SUBMITTER: Yamada S
PROVIDER: S-EPMC7263140 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Yamada Shintaro S Hinch Anjali Gupta AG Kamido Hisashi H Zhang Yongwei Y Edelmann Winfried W Keeney Scott S
Genes & development 20200430 11-12
Exonucleolytic resection, critical to repair double-strand breaks (DSBs) by recombination, is not well understood, particularly in mammalian meiosis. Here, we define structures of resected DSBs in mouse spermatocytes genome-wide at nucleotide resolution. Resection tracts averaged 1100 nt, but with substantial fine-scale heterogeneity at individual hot spots. Surprisingly, EXO1 is not the major 5' → 3' exonuclease, but the DSB-responsive kinase ATM proved a key regulator of both initiation and ex ...[more]