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Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging.

ABSTRACT: This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1??mm3 volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /s?p?/) microscope presented here, we demonstrate cellular resolution imaging at depths exceeding 330 ?m in optically scattering mouse brain samples and dendritic imaging in more superficial layers.

SUBMITTER: Kumar M

PROVIDER: S-EPMC7263705 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging.

Kumar Manish M Kozorovitskiy Yevgenia Y

Optics letters 20190401 7

This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 <i>mm</i><sup>3</sup> volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōp ...[more]

PMID: 30933127

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