Ontology highlight
ABSTRACT:
SUBMITTER: Kumar M
PROVIDER: S-EPMC7263705 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Kumar Manish M Kozorovitskiy Yevgenia Y
Optics letters 20190401 7
This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in single objective light-sheet microscopy. We show undistorted 3D imaging spanning complete zebrafish larvae and over 1 <i>mm</i><sup>3</sup> volumes for thick mouse brain sections. We use remote galvo scanning for light-sheet creation and develop a processing pipeline for 3D tiling across different axes. With the improved one photon (1p) tilt-invariant scanned oblique plane illumination (SOPi, /sōp ...[more]