Ontology highlight
ABSTRACT:
SUBMITTER: Cao J
PROVIDER: S-EPMC7264055 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Cao Jingxin J Layne Christine C Varga Jessie J Deschambault Yvon Y
MethodsX 20200515
Vaccinia virus is capable of replicating in a wide range of vertebrate animal cells. However, deletion of the two virus host range genes, E3L and K3L, would render replication of the virus abortive in all the cell types tested, except the cells with defective PKR and RNase L activity. By expressing different poxvirus K3 ortholog proteins in the E3L and K3L double knockout vaccinia virus, we can construct a mutant vaccinia virus with modified host range. Here, using poxvirus K3 ortholog as a posi ...[more]