Ontology highlight
ABSTRACT:
SUBMITTER: Eskandari-Shahraki M
PROVIDER: S-EPMC7265374 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Eskandari-Shahraki Marzieh M Prud'homme Bruno B Bergeron Francis F Manjunath Puttaswamy P
Scientific reports 20200602 1
The binder of sperm family of proteins has been reported to be indispensable for sperm maturation and capacitation. However, their physiological functions in fertility have only been studied in vitro. CRISPR/Cas9 genome editing was utilized to generate double knockout (DKO) mice by simultaneously targeting the two murine binder of sperm genes, Bsph1 and Bsph2. To confirm that the homologous genes and proteins were completely eliminated in the DKO mice, different methods such as reverse transcrip ...[more]