Ontology highlight
ABSTRACT:
SUBMITTER: Eisen TJ
PROVIDER: S-EPMC7265681 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Eisen Timothy J TJ Eichhorn Stephen W SW Subtelny Alexander O AO Lin Kathy S KS McGeary Sean E SE Gupta Sumeet S Bartel David P DP
Molecular cell 20200102 4
For all but a few mRNAs, the dynamics of metabolism are unknown. Here, we developed an experimental and analytical framework for examining these dynamics for mRNAs from thousands of genes. mRNAs of mouse fibroblasts exit the nucleus with diverse intragenic and intergenic poly(A)-tail lengths. Once in the cytoplasm, they have a broad (1000-fold) range of deadenylation rate constants, which correspond to cytoplasmic lifetimes. Indeed, with few exceptions, degradation appears to occur primarily thr ...[more]