Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants.

ABSTRACT: Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tolerance, while variegated Pelargonium zonale has been proven to serve as an excellent model for the metabolite allocation between sink tissue and source tissue within the same organ. However, the genomes of these plants are still not sequenced, limiting their application in molecular studies. To investigate the transcript abundance by next-generation sequencing, high-quality RNA input is required. Leaves of both P. zonale and R. serbica are rich in polyphenols that interfere with high-quality RNA extraction by common protocols. Moreover, low water content and high amount of sugars and other osmoprotectants in desiccated R. serbica leaves present the additiona

SUBMITTER: Vidovic M 

PROVIDER: S-EPMC7266862 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature