Lipid-associated PML structures assemble nuclear lipid droplets containing CCT? and Lipin1.

ABSTRACT: Nuclear lipid droplets (nLDs) form on the inner nuclear membrane by a mechanism involving promyelocytic leukemia (PML), the protein scaffold of PML nuclear bodies. We report that PML structures on nLDs in oleate-treated U2OS cells, referred to as lipid-associated PML structures (LAPS), differ from canonical PML nuclear bodies by the relative absence of SUMO1, SP100, and DAXX. These nLDs were also enriched in CTP:phosphocholine cytidylyltransferase ? (CCT?), the phosphatidic acid phosphatase Lipin1, and DAG. Translocation of CCT? onto nLDs was mediated by its ?-helical M-domain but was not correlated with its activator DAG. High-resolution imaging revealed that CCT? and LAPS occupied distinct polarized regions on nLDs. PML knockout U2OS (PML KO) cells lacking LAPS had a 40-50% reduction in nLDs with associated CCT?, and residual nLDs were almost devoid of Lipin1 and DAG. As a result, phosphatidylcholine and triacylglycerol synthesis was inhibited in PML KO cells. We conclude that in response to excess exogenous fatty acids, LAPS are required to assemble nLDs that are competent to recruit CCT? and Lipin1.

SUBMITTER: Lee J 

PROVIDER: S-EPMC7266991 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature