Unknown

Dataset Information

0

Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium.


ABSTRACT: The ventricular epithelium of the adult forebrain is a heterogeneous cell population that is a source of both quiescent and activated neural stem cells (qNSCs and aNSCs, respectively). We genetically targeted a subset of ventricle-contacting, glial fibrillary acidic protein (GFAP)-expressing cells, to study their involvement in qNSC/aNSC-mediated adult neurogenesis. Ventricle-contacting GFAP+ cells were lineage-traced beginning in early adulthood using adult brain electroporation and produced small numbers of olfactory bulb neuroblasts until at least 21 mo of age. Notably, electroporated GFAP+ neurogenic precursors were distinct from both qNSCs and aNSCs: they did not give rise to neurosphere-forming aNSCs in vivo or after extended passaging in vitro and they were not recruited during niche regeneration. GFAP+ cells with these properties included a FoxJ1+GFAP+ subset, as they were also present in an inducible FoxJ1 transgenic lineage-tracing model. Transiently overexpressing Mash1 increased the neurogenic output of electroporated GFAP+ cells in vivo, identifying them as a potentially recruitable population. We propose that the qNSC/aNSC lineage of the adult forebrain coexists with a distinct, minimally expanding subset of GFAP+ neurogenic precursors.

SUBMITTER: Joppe SE 

PROVIDER: S-EPMC7266992 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3711666 | biostudies-literature
| S-EPMC6782621 | biostudies-literature
| S-EPMC3361692 | biostudies-literature
| S-EPMC2588566 | biostudies-literature
| S-EPMC2714762 | biostudies-literature
| S-EPMC7077007 | biostudies-literature
| S-EPMC2613692 | biostudies-literature
| PRJNA556434 | ENA
| S-EPMC2861434 | biostudies-literature
2021-02-10 | GSE165555 | GEO