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Data on the isolation and identification of thermotolerant microorganisms from cow manure promising for organic waste processing.

ABSTRACT: Thermotolerant microorganisms play an essential role in the composting process of organic waste; they are responsible for the degradation process of organic compounds owing to their enzymatic activity in the high-temperature phase of composting. This article presents data on the isolation and identification of thermotolerant microorganisms from cattle manure. In total, three bacterial strains with the ability to grow at 50°C were isolated on different media. The cultural and morphological characteristics of the strains are presented. By analysis of the 16S rRNA gene sequence, the isolates were assigned to the species Bacillus coagulans and Bacillus licheniformis. Isolated strains were characterized for their enzymatic potential (lipolytic, amylolytic, and proteolytic). These results can be promising for further studies in order to design biological additive for accelerated composting of organic waste.

SUBMITTER: Moldagulova N

PROVIDER: S-EPMC7267706 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Data on the isolation and identification of thermotolerant microorganisms from cow manure promising for organic waste processing.

Moldagulova Nazira N Ayupova Aigul A Sembayeva Dinara D Duambekov Musagali M Khassenova Elmira E Nagyzbekkyzy Elvira E Danlybayeva Gaziza G Sarsenova Ainur A

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Thermotolerant microorganisms play an essential role in the composting process of organic waste; they are responsible for the degradation process of organic compounds owing to their enzymatic activity in the high-temperature phase of composting. This article presents data on the isolation and identification of thermotolerant microorganisms from cattle manure. In total, three bacterial strains with the ability to grow at 50°C were isolated on different media. The cultural and morphological charac ...[more]

PMID: 32514418

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Project description:Application of manure from antibiotic-treated animals to crops facilitates the dissemination of antibiotic resistance determinants into the environment. However, our knowledge of the identity, diversity, and patterns of distribution of these antibiotic resistance determinants remains limited. We used a new combination of methods to examine the resistome of dairy cow manure, a common soil amendment. Metagenomic libraries constructed with DNA extracted from manure were screened for resistance to beta-lactams, phenicols, aminoglycosides, and tetracyclines. Functional screening of fosmid and small-insert libraries identified 80 different antibiotic resistance genes whose deduced protein sequences were on average 50 to 60% identical to sequences deposited in GenBank. The resistance genes were frequently found in clusters and originated from a taxonomically diverse set of species, suggesting that some microorganisms in manure harbor multiple resistance genes. Furthermore, amid the great genetic diversity in manure, we discovered a novel clade of chloramphenicol acetyltransferases. Our study combined functional metagenomics with third-generation PacBio sequencing to significantly extend the roster of functional antibiotic resistance genes found in animal gut bacteria, providing a particularly broad resource for understanding the origins and dispersal of antibiotic resistance genes in agriculture and clinical settings. IMPORTANCE The increasing prevalence of antibiotic resistance among bacteria is one of the most intractable challenges in 21st-century public health. The origins of resistance are complex, and a better understanding of the impacts of antibiotics used on farms would produce a more robust platform for public policy. Microbiomes of farm animals are reservoirs of antibiotic resistance genes, which may affect distribution of antibiotic resistance genes in human pathogens. Previous studies have focused on antibiotic resistance genes in manures of animals subjected to intensive antibiotic use, such as pigs and chickens. Cow manure has received less attention, although it is commonly used in crop production. Here, we report the discovery of novel and diverse antibiotic resistance genes in the cow microbiome, demonstrating that it is a significant reservoir of antibiotic resistance genes. The genomic resource presented here lays the groundwork for understanding the dispersal of antibiotic resistance from the agroecosystem to other settings.

Project description:The continuous cropping barrier is an important factor leading to the decline of watermelon quality and yield. In this study, we focused on a bio-organic fertilizer prepared with one bacterial strain, Bacillus sp. XG-1, to prevent the occurrence of the continuous cropping barrier. The strain XG-1 was isolated from watermelon rhizosphere soil, and promoted the growth of watermelon by producing phytase (0.19 U/mL), indole-3-acetic acid (IAA, 7.31 mg/L), and gibberellins (GA3, 2.47 mg/L). In addition, the strain also possessed a strong antagonistic effect against the pathogen Fusarium oxysporum f. sp. niveum (Fon) by inhibiting conidia germination with an inhibition ratio of 85.3% and mycelium growth. The bio-organic fertilizer fermented by XG-1, based on cow manure compost and rapeseed meal (85:15, w/w) under optimal conditions, was mixed in soil (watermelon had been planted for two consecutive years). After the cultivation of watermelon for 50 d, a higher density of XG-1 (9.79 × 105 colony-forming units (CFU)/g) and one order of magnitude lower of Fon (1.29 × 103 copies/g) were detected in the rhizosphere soil compared with soils without bio-organic fertilizer (7.59 × 104 copies/g for Fon), leading to an 86.4% control efficiency of watermelon caused by Fusarium wilt. The application of bio-organic fertilizer enriched soil nutrients, including the organic matter (13.2%), total nitrogen (13.9%), total phosphorus (20.5%), and total potassium (3.77%), adjusted the soil pH from 6.69 to 7.01, and significantly improved the watermelon growth in terms of the seedling height, root length, fresh weight of seedling and root with increase of 78.8%, 72.2%, 84.6%, and 96.4%, respectively. This study regarded the watermelon continuous cropping soil as the research point, and focused on inhibiting Fon, regulating soil properties and enhancing watermelon growth to eliminate the continuous cropping barrier through a combination of compost and functional strains, demonstrating the potential application value in watermelon production.

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Data on the isolation and identification of thermotolerant microorganisms from cow manure promising for organic waste processing.

Publications

Data on the isolation and identification of thermotolerant microorganisms from cow manure promising for organic waste processing.

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