<i>In Vivo</i> Tracking of Adoptively Transferred Natural Killer Cells in Rhesus Macaques Using ⁸⁹Zirconium-Oxine Cell Labeling and PET Imaging.

ABSTRACT:

Purpose

Trials of adoptive natural killer (NK)-cell immunotherapy for hematologic malignancies have thus far shown only marginal effects, despite the potent in vitro antitumor activity of these cells. Homing of infused cells to tumor microenvironments is critical for efficacy, but has not been well characterized. We established a novel method to track and quantify the distribution of adoptively transferred NK cells using rhesus macaques (RM) as a clinically relevant preclinical model.

Experimental design

RM NK cells were expanded ex vivo for 14-21 days, labeled with ⁸⁹Zr-oxine complex, and assessed for phenotype, function, and survival. Trafficking of ⁸⁹Zr-labeled ex vivo-expanded NK cells infused into RMs was monitored and quantita

SUBMITTER: Sato N 

PROVIDER: S-EPMC7269806 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature