Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell via GSK-3? and ?-Catenin.

ABSTRACT: Objectives:To demonstrate the effect of Ginsenoside Rg1 on the differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Subsequently, a rational mechanism for the detection of Rg1 which affects mesenchymal stem cell differentiation was explored. Methods:Flow cytometry is used for cell identification. The differentiation ability of hBM-MSCs was studied by differentiation culture. SA-?-gal staining is used to detect cell senescence levels. Western blot and immunofluorescence were used to determine protein expression levels. RT-qPCR is used to detect mRNA expression levels. Results:Rg1 regulates the differentiation of hBM-MSCs. Differentiation culture analysis showed that Rg1 promoted cells to osteogenesis and chondrogenesis. Western blot results showed that Rg1 regulated the overactivation of the ?-catenin signaling pathway and significantly adjusted the phosphorylation of GSK-3?. GSK-3? inhibitor (Licl) significantly increased Rg1-induced phosphorylation of GSK-3?, which in turn reduced Rg1-induced differentiation of hBM-MSCs. Conclusion:Ginsenoside Rg1 can reduce the excessive activation of the Wnt pathway in senescent cells by inhibiting the phosphorylation of GSK-3? and regulate the mesenchymal stem cell differentiation ability.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7271209 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature