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HEATR1 Deficiency Promotes Chemoresistance via Upregulating ZNF185 and Downregulating SMAD4 in Pancreatic Cancer.

ABSTRACT:

Objective

To discover the correlated gene with HEATR1 in regulating chemoresistance of gemcitabine.

Methods

Gene chip analysis was performed to find out differential genes between HEATR1-KD and control groups. The top 20 genes were subjected to high-content screening, and functional assay was implemented. Gene expression profiling was carried out to find the downstream target. Immunohistochemistry and survival analysis were performed.

Results

ZNF185 fold change (4.5285) was the most significant between the HEATR1-KD and control groups. Knocking down ZNF185 could promote the chemosensitivity, apoptosis, and proliferative inhibition, with SMAD4 significantly upregulated. Patients with high HEATR1 and SMAD4 or low ZNF185 exhibited better survival.

Conclusion

HEATR1, ZNF185, and SMAD4 could affect the chemosensitivity of gemcitabine and may be the indicators of gemcitabine selection in the chemotherapy of pancreatic cancer.

SUBMITTER: Fang Y

PROVIDER: S-EPMC7271247 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

HEATR1 Deficiency Promotes Chemoresistance via Upregulating ZNF185 and Downregulating SMAD4 in Pancreatic Cancer.

Fang Yuan Y Han Xu X Li Jianang J Kuang Tiantao T Lou Wenhui W

Journal of oncology 20200526

<h4>Objective</h4>To discover the correlated gene with HEATR1 in regulating chemoresistance of gemcitabine.<h4>Methods</h4>Gene chip analysis was performed to find out differential genes between HEATR1-KD and control groups. The top 20 genes were subjected to high-content screening, and functional assay was implemented. Gene expression profiling was carried out to find the downstream target. Immunohistochemistry and survival analysis were performed.<h4>Results</h4>ZNF185 fold change (4.5285) was ...[more]

PMID: 32565799

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