Ontology highlight
ABSTRACT:
SUBMITTER: Esposito D
PROVIDER: S-EPMC7271859 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Protein expression and purification 20200604
The SARS-CoV-2 spike trimer is the primary antigen for several serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Until stable cell lines are developed to increase the titer of this secreted protein in mammalian cell culture, the low yield of spike protein produced from transient transfection of HEK293 cells will be a limiting factor for these assays. To improve the yield of spike protein and support the high demand for antigens in serology assays, we in ...[more]