Ontology highlight
ABSTRACT:
SUBMITTER: Garrett-Thomson SC
PROVIDER: S-EPMC7272049 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Garrett-Thomson Sarah C SC Massimi Aldo A Fedorov Elena V EV Bonanno Jeffrey B JB Scandiuzzi Lisa L Hillerich Brandan B Seidel Ronald D RD Love James D JD Garforth Scott J SJ Guha Chandan C Almo Steven C SC
PloS one 20200604 6
The B7 family represents one of the best-studied subgroups within the Ig superfamily, yet new interactions continue to be discovered. However, this binding promiscuity represents a major challenge for defining the biological contribution of each specific interaction. We developed a strategy for addressing these challenges by combining cell microarray and high-throughput FACS methods to screen for promiscuous binding events, map binding interfaces, and generate functionally selective reagents. Ap ...[more]