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De novo RNA sequencing analysis of Aeluropus littoralis halophyte plant under salinity stress.


ABSTRACT: The study of salt tolerance mechanisms in halophyte plants can provide valuable information for crop breeding and plant engineering programs. The aim of the present study was to investigate whole transcriptome analysis of Aeluropus littoralis in response to salinity stress (200 and 400?mM NaCl) by de novo RNA-sequencing. To assemble the transcriptome, Trinity v2.4.0 and Bridger tools, were comparatively used with two k-mer sizes (25 and 32?bp). The de novo assembled transcriptome by Bridger (k-mer 32) was chosen as final assembly for subsequent analysis. In general, 103290 transcripts were obtained. The differential expression analysis (log2FC?>?1 and FDR?

SUBMITTER: Younesi-Melerdi E 

PROVIDER: S-EPMC7272644 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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De novo RNA sequencing analysis of Aeluropus littoralis halophyte plant under salinity stress.

Younesi-Melerdi Elham E   Nematzadeh Ghorban-Ali GA   Pakdin-Parizi Ali A   Bakhtiarizadeh Mohammad Reza MR   Motahari Seyed Abolfazl SA  

Scientific reports 20200604 1


The study of salt tolerance mechanisms in halophyte plants can provide valuable information for crop breeding and plant engineering programs. The aim of the present study was to investigate whole transcriptome analysis of Aeluropus littoralis in response to salinity stress (200 and 400 mM NaCl) by de novo RNA-sequencing. To assemble the transcriptome, Trinity v2.4.0 and Bridger tools, were comparatively used with two k-mer sizes (25 and 32 bp). The de novo assembled transcriptome by Bridger (k-m  ...[more]

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