Dataset Information

Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa.

ABSTRACT: Otitis externa is one of the most common diseases in dogs. It is associated with bacteria and yeast, which are regarded as secondary causes. Cerumen is a biological substance playing an important role in the protection of ear skin. The involvement of cerumen in immune defense is poorly understood. MicroRNAs can modulate the host immune response and can provide promising biomarkers for several inflammatory and infectious disorder diagnosis. The aims of this study were to profile the cerumen miRNA signature associated with otitis externa in dogs, integrate miRNAs to their target genes related to immune functions, and investigate their potential use as biomarkers. Cerumen was collected from healthy and otitis affected dogs and the expression of miRNAs was profiled by Next Generation Sequencing; the validation of the altered miRNAs was performed using RT-qPCR. The potential ability of miRNAs to modulate immune-related genes was investigated using bioinformatics tools. The results pointed out that 32 miRNAs, of which 14 were up- and 18 down-regulated, were differentially expressed in healthy vs. otitis-affected dogs. These results were verified by RT-qPCR. To assess the diagnostic value of miRNAs, ROC analysis was carried out, highlighting that 4 miRNAs are potential biomarkers to discriminate otitis-affected dogs. Bioinformatics showed that cerumen miRNAs may be involved in the modulation of host immune response. In conclusion, we have demonstrated for the first time that miRNAs can be efficiently extracted and quantified from cerumen, that their profile changes between healthy and otitis affected dogs, and that they may serve as potential biomarkers. Further studies are necessary to confirm their diagnostic value and to investigate their interaction with immune-related genes.

SUBMITTER: Lecchi C

PROVIDER: S-EPMC7273745 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa.

Lecchi Cristina C Zamarian Valentina V Borriello Giorgia G Galiero Giorgio G Grilli Guido G Caniatti Mario M D'Urso Elisa Silvia ES Roccabianca Paola P Perego Roberta R Minero Michela M Legnani Sara S Calogero Raffaele R Arigoni Maddalena M Ceciliani Fabrizio F

Frontiers in immunology 20200529

Otitis externa is one of the most common diseases in dogs. It is associated with bacteria and yeast, which are regarded as secondary causes. Cerumen is a biological substance playing an important role in the protection of ear skin. The involvement of cerumen in immune defense is poorly understood. MicroRNAs can modulate the host immune response and can provide promising biomarkers for several inflammatory and infectious disorder diagnosis. The aims of this study were to profile the cerumen miRNA ...[more]

PMID: 32547539

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Project description:BACKGROUND:Canine otitis externa is a painful condition which can be challenging to treat due to difficulties in the administration of otic medication. This can be due to lack of owner compliance in the application of ear drops or due to the resentment that some dogs demonstrate when attempts are made to administer topical medication into a sensitive ear canal. The aim of the study was to assess the efficacy of a topical LED-illuminated gel (LIG) in canine otitis externa in comparison to standard of care therapy. Dogs with spontaneous otitis externa were randomly allocated in three groups: groups QW received LIG once weekly; BW received LIG twice weekly; group C received enrofloxacin and silver sulfadiazine twice daily. LIG consists of a topical application of a gel containing chromophores that, when illuminated by a LED lamp, re-emit fluorescent light which can stimulate physiological responses, promoting healing and controlling bacteria. The evaluation protocol (T0 to T5) considered clinical assessment (OTIS-3-index-scoring-system; pruritus-severity-scale; pain-severity-score; aural temperature), cytological scoring system, quali-quantitative bacteriologic assessment. RESULTS:All groups (QW, n =?21; BW, n =?23; C, n =?20) showed improvement during the study (QW: P <?0.02 for cytological and pain scores, P <?0.003 for bacteriologic assessment, P <?10-?4 for pruritus, total OTIS-3 and temperature assessments; BW: P <?10-?4 for all clinical, cytological and bacteriologic assessments; C: P <?0.02 for all clinical and cytological assessments, P <?10-?4 for bacteriologic assessment). The highest clinical score reduction occurred in Group BW (P <?0.014 in T3; P <?0.001 in T4 and P <?10-?4 in T5). BW reached the clinically relevant effect level at T3 (-?3.26?±?1.21 levels), QW reached it at T4 (-?3.24?±?0.99), C did not reach it. No differences between groups were seen in the reduction of CFU/mL (T0-T5). CONCLUSIONS:All treatment groups showed a positive clinical effect. LIG administered twice-a-week was the most favourable protocol of the study. LIG may be considered beneficial in the management of canine otitis externa; it seems to be effective in controlling the clinical condition, including the signs of inflammation and local pain, the bacterial growth, and it may help increasing treatment compliance.

Dataset Information

Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa.

Publications

Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa.

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