Ontology highlight
ABSTRACT:
SUBMITTER: Mays ZJ
PROVIDER: S-EPMC7274816 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Mays Zachary Js ZJ Mohan Karishma K Trivedi Vikas D VD Chappell Todd C TC Nair Nikhil U NU
Chemical communications (Cambridge, England) 20200409 39
There is broad interest in engineering phenylalanine ammonia-lyase (PAL) for its biocatalytic applications in industry and medicine. While site-specific mutagenesis has been employed to improve PAL stability or substrate specificity, combinatorial techniques are poorly explored. Here, we report development of a directed evolution technique to engineer PAL enzymes. Central to this approach is a high-throughput enrichment that couples E. coli growth to PAL activity. Starting with the PAL used in t ...[more]