Rapid analysis of ricin using hot acid digestion and MALDI-TOF mass spectrometry.
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ABSTRACT:
SUBMITTER: Chen D
PROVIDER: S-EPMC7278220 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Publications
Rapid analysis of ricin using hot acid digestion and MALDI-TOF mass spectrometry.
Chen Dapeng D Bryden Wayne A WA Fenselau Catherine C
Journal of mass spectrometry : JMS 20180815 10
Ricin is a protein toxin of considerable interest in forensics. A novel strategy is reported here for rapid detection of ricin based on microwave-assisted hot acid digestion and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. Ricin samples are subjected to aspartate-selective hydrolysis, and biomarker peptide products are characterized by mass spectrometry. Spectra are obtained using post source decay and searched against a protein database. Several advantages are o ...[more]
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