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Heterologous Production of Lasso Peptide Capistruin in a Burkholderia Host.


ABSTRACT: Burkholderia bacteria are an emerging source of natural products with applications in agriculture and medicine. The heterologous expression of biosynthetic gene clusters can streamline natural product discovery; however, production yields with the commonly used Escherichia coli host are usually low. Following the current paradigm that one host does not fit all, we aim to develop a Burkholderia host to ultimately tap into the biosynthetic potential of Burkholderia genomes, which can contain up to 27 biosynthetic gene clusters per genome. Because a close phylogenetic relationship is expected to improve the odds of success due to compatible gene expression and precursor supply, we tested Burkholderia sp. FERM BP-3421, a nonpathogenic isolate previously used to produce natural products at industrial scales. We show here that FERM BP-3421 can produce the model lasso peptide capistruin in yields that are at least 65 times and up to 580 times higher than the previously used E. coli host.

SUBMITTER: Kunakom S 

PROVIDER: S-EPMC7278363 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Heterologous Production of Lasso Peptide Capistruin in a <i>Burkholderia</i> Host.

Kunakom Sylvia S   Eustáquio Alessandra S AS  

ACS synthetic biology 20200113 2


<i>Burkholderia</i> bacteria are an emerging source of natural products with applications in agriculture and medicine. The heterologous expression of biosynthetic gene clusters can streamline natural product discovery; however, production yields with the commonly used <i>Escherichia coli</i> host are usually low. Following the current paradigm that one host does not fit all, we aim to develop a <i>Burkholderia</i> host to ultimately tap into the biosynthetic potential of <i>Burkholderia</i> geno  ...[more]

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