Use of the HRM Method in Quick Identification of FecXO Mutation in Highly Prolific Olkuska Sheep.
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ABSTRACT:
SUBMITTER: Smolucha G
PROVIDER: S-EPMC7278464 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
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Use of the HRM Method in Quick Identification of FecX<sup>O</sup> Mutation in Highly Prolific Olkuska Sheep.
Smołucha Grzegorz G Piórkowska Katarzyna K Ropka-Molik Katarzyna K Sikora Jacek J
Animals : an open access journal from MDPI 20200514 5
Olkuska is a highly prolific sheep breed in Poland. Thanks to earlier identification of the genetic basis of its prolificacy, a mutation in the <i>BMP-15</i> gene, we can use molecular biology tools to identify this causative mutation affecting prolificacy. In our research, we used the High-Resolution Melting (HRM) and Sanger sequencing methods to identify the genotypes of the studied animals. The result obtained by the HRM method is identical to those obtained by the sequencing method, which co ...[more]
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