Crystallization and X-ray analysis of Borrelia burgdorferi β-barrel assembly machinery A.

ABSTRACT: Mitochondria, chloroplasts and several species of bacteria have outer membrane proteins (OMPs) that perform many essential biological functions. The β-barrel assembly machinery (BAM) complex is one of the OMPs of Borrelia burgdorferi, the pathogenic spirochete that causes Lyme disease, and its BamA component (BbBamA) includes a C-terminal β-barrel domain and five N-terminal periplasmic polypeptide-transport-associated (POTRA) domains, which together perform a central transport function. In the current work, the production, crystallization and X-ray analysis of the three N-terminal POTRA domains of BbBamA (BbBamA-POTRA P1-P3; residues 30-273) were carried out. The crystals of BbBamA-POTRA P1-P3 belonged to space group P2₁, with unit-cell parameters a = 45.353, b = 111.538, c = 64

SUBMITTER: Dong S 

PROVIDER: S-EPMC7278503 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature