Dataset Information

Effect of Diet on the Vitamin B Profile of Bovine Milk-Based Protein Ingredients.

ABSTRACT: The influence of diet on the water-soluble vitamin composition of skim milk powder and whey protein ingredients produced from the milk of cows fed pasture or concentrate-based diets was examined. Fifty-one Holstein-Friesian cows were randomly assigned into three diets (n=17) consisting of outdoor grazing of perennial ryegrass (GRS), perennial ryegrass/white clover (CLV), or indoor feeding of total mixed ration (TMR) for an entire lactation. Raw mid-lactation milk from each group was processed into skim milk powder and further processed to yield micellar casein whey and acid whey. Sweet whey was also produced by renneting of pasteurised whole milk from each system. The water-soluble vitamin profile of each sample was analysed using a combination of direct injection mass spectrometry and reverse-phase liquid chromatography-mass spectrometry. Vitamin B3 and B3-amide concentrations were significantly higher (p < 0.05) in TMR-derived samples than in those from CLV and GRS, respectively. Vitamin B1, B2, and B7 concentrations were significantly higher in GRS and CLV-derived samples than those from TMR. Significant differences in vitamins B1, B2, and B3-amide were also observed between protein ingredient types. This study indicates that bovine feeding systems have a significant effect on B vitamin composition across a range of protein ingredient types.

SUBMITTER: Magan JB

PROVIDER: S-EPMC7278826 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Effect of Diet on the Vitamin B Profile of Bovine Milk-Based Protein Ingredients.

Magan Jonathan B JB O'Callaghan Tom F TF Zheng Jiamin J Zhang Lun L Mandal Rupasri R Hennessy Deirdre D Fenelon Mark A MA Wishart David S DS Kelly Alan L AL McCarthy Noel A NA

Foods (Basel, Switzerland) 20200504 5

The influence of diet on the water-soluble vitamin composition of skim milk powder and whey protein ingredients produced from the milk of cows fed pasture or concentrate-based diets was examined. Fifty-one Holstein-Friesian cows were randomly assigned into three diets (<i>n</i>=17) consisting of outdoor grazing of perennial ryegrass (GRS), perennial ryegrass/white clover (CLV), or indoor feeding of total mixed ration (TMR) for an entire lactation. Raw mid-lactation milk from each group was proce ...[more]

PMID: 32375412

Similar Datasets

Project description:The influence of bovine diet on the metabolome of reconstituted skim milk powder (SMP) and protein ingredients produced from the milk of cows fed on pasture or concentrate-based diets was investigated. Cows were randomly assigned to diets consisting of perennial ryegrass only (GRS), perennial ryegrass/white clover sward (CLV), or indoor total mixed ration (TMR) for an entire lactation. Raw milk obtained from each group was processed at pilot scale, to produce SMP and sweet whey, and SMP was further processed at laboratory scale, to yield ideal whey and acid whey. The total amino acid composition and metabolome of each sample were analyzed, using high-performance cation exchange and a targeted combination of direct-injection mass spectrometry and reverse-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively. The nitrogen composition of the products from each of the diets was similar, with one exception being the significantly higher nonprotein nitrogen content in TMR-derived skim milk powder than that from the GRS system. Total amino acid analysis showed significantly higher concentrations of glycine in GRS- and CLV-derived sweet whey and acid whey than in those from TMR. The cysteine contents of CLV-derived ideal whey and acid whey were significantly higher than for TMR, while the valine content of GRS-derived acid whey was significantly higher than TMR. The phenylalanine content of GRS-derived ideal whey was significantly higher than that from CLV. Metabolomic analysis showed significantly higher concentrations of the metabolites glutamine, valine, and phosphocreatine in each ingredient type derived from TMR than those from GRS or CLV, while the serine content of each GRS-derived ingredient type was significantly higher than that in TMR-derived ingredients. These results demonstrate that the type of bovine feeding system used can have a significant effect on the amino acid composition and metabolome of skim milk and whey powders and may aid in the selection of raw materials for product manufacture, while the clear separation between the samples gives further evidence for distinguishing milk products produced from different feeding systems based on LC-MS/MS.

Project description:The use of glycerin in diets for dairy cows initially emerged as an alternative for the prevention and control of ketosis. However, despite some controversy, there are still several studies associating glycerin with increases in daily milk yield, with possible changes in its constituents. Therefore, the objective of this study was to evaluate, using a meta-analysis approach, the effect of glycerin inclusion in dairy cow diets on milk fatty acid. Twenty-two peer-reviewed publications with 66 treatment means were included in data set. The effect of glycerin inclusion in diet (treatment) were evaluated using random-effect models to examine the weighted mean differences (WMD) between a control diet (without glycerin in the diet) and the treatment diet. Heterogeneity was explored by meta-regression and subgroup analysis performed for: genetic type; days in milk; experimental period; glycerin in diet; glycerin type and concentrate in diet. Inclusion of glycerin in the diet increased the digestibility of dry matter and protein, as well as ruminal propionate. It did not affect dry matter intake (P = 0.351) and milk yield (P = 0.730). The effect of glycerin inclusion on the milk fat yield is dependent on the genetic group, in which Holstein (WMD = -0.04 kg/d; P = 0.010) and Holstein-crossbreed (WMD = -0.10 kg/d; P < 0.0001) cows produced less fat in milk compared to Jersey cows, when glycerin was included in the diets. Glycine inclusions of up to 100 g/kg in the diet of dairy cows did not negatively affect milk production and composition. However, inclusions above 150 g/kg of glycerin in the diet reduced the concentration of fat, and of unsaturated, monounsaturated, polyunsaturated fatty acids and conjugated linoleic acid (CLA C18: 2 cis-9 and trans-11) in milk. The results reported in our meta-analysis does not demonstrate the effectiveness of glycerin in improving the composition of milk and a group of fatty acids of importance for human health such as C18: 2 cis-9, trans-11 CLA.

Dataset Information

Effect of Diet on the Vitamin B Profile of Bovine Milk-Based Protein Ingredients.

Publications

Effect of Diet on the Vitamin B Profile of Bovine Milk-Based Protein Ingredients.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets