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Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase.


ABSTRACT: Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as Sphingobium sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by Sphingobium sp. YC-JY1. Escherichia coli BL21 (DE3) cells harboring pET28a-bisdAB achieved the ability to degrade BPA. The bisdB gene knockout strain YC-JY1?bisdB was unable to degrade BPA indicating that P450bisdB was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.

SUBMITTER: Jia Y 

PROVIDER: S-EPMC7278973 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Biodegradation of Bisphenol A by <i>Sphingobium</i> sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase.

Jia Yang Y   Eltoukhy Adel A   Wang Junhuan J   Li Xianjun X   Hlaing Thet Su TS   Aung Mar Mar MM   Nwe May Thet MT   Lamraoui Imane I   Yan Yanchun Y  

International journal of molecular sciences 20200519 10


Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as <i>Sphingobium</i> sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by <i>Sphing  ...[more]

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