Ontology highlight
ABSTRACT:
SUBMITTER: Olmedo-Suarez MA
PROVIDER: S-EPMC7281659 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Olmedo-Suárez Miguel Ángel MÁ Sekiguchi Tomohiro T Takano Atsushi A Cañizares-Macías Maria Del Pilar MDP Futai Nobuyuki N
Micromachines 20200430 5
We developed a portable device made of poly(dimethylsiloxane) (PDMS)/polymethylmethacrylate (PMMA) for long-term 3D cell culture of vascular endothelial cells for the development of a vascular network and evaluated the device under different transitions between normoxia and hypoxia with good optical accessibility. The combination of a nested reservoir device and a bicarbonate/ascorbate buffer system accomplished on-chip incubation with 4.91 ± 0.86% pO<sub>2</sub> and 5.19 ± 1.70% pCO<sub>2</sub> ...[more]