Ontology highlight
ABSTRACT:
SUBMITTER: Bao C
PROVIDER: S-EPMC7282821 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Bao Chen C Loerch Sarah S Ling Clarence C Korostelev Andrei A AA Grigorieff Nikolaus N Ermolenko Dmitri N DN
eLife 20200519
Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) experiments, we studied how frameshift-inducing stem-loops from <i>E. coli dnaX</i> mRNA and the <i>gag-pol</i> transcript of Human Immunodeficiency Virus (HIV) perturb translation elo ...[more]