Ontology highlight
ABSTRACT:
SUBMITTER: Schleicher L
PROVIDER: S-EPMC7283201 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Schleicher Lena L Fritz Günter G Seifert Jana J Steuber Julia J
Archives of microbiology 20200118 5
Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na<sup>+</sup>-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protein yield after oxic and anoxic cell disruption by the EmulsiFlex, by ultrasonication, and by glass beads treatment. With an overall membrane protein yield of 50 mg L<sup>- ...[more]