Dataset Information

Field Screening of Rice Germplasm (Oryza sativa L. ssp. japonica) Based on Days to Flowering for Drought Escape.

ABSTRACT: Terminal drought stress is one of the restrictive factors in rice production and is expected to upsurge under the current situation of climate change. The study evaluated the performance of 2030 rice genotypes under continuous drought stress conditions based on days to flowering (DF). The genotypes under augmented randomized complete block design were sown in May/June of 2017 and 2018 in the field with movable rainout that resulted in huge genetic diversity among the accessions. Descriptive statistics confirmed clear variation among accessions on growth duration, plant height to leaf, plant height to panicle, and germination percentage. Correlation, chemometric, and agglomerative hierarchical cluster analyses were performed that categorized the germplasm into 10 groups. Genotypes in clusters VIII and IX (drought-resistant) revealed better agronomic performance in terms of reduced days to flowering, but conversely taller plant height and higher maturity (%) under severe stress. Genotypes in clusters IV, V, and X were discovered to be drought-susceptible. The screened genotypes like Longjing 12, Longdun 102, Yanjing 22, Liaojing 27, Xiaohongbandao, Songjing 17, and Zaoshuqingsen can be utilized in rice breeding improvement programs for drought tolerance in terms of severe continuous drought, as well as terminal drought stress.

SUBMITTER: Ahmad MS

PROVIDER: S-EPMC7284638 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Field Screening of Rice Germplasm (<i>Oryza sativa</i> L. ssp. <i>japonica</i>) Based on Days to Flowering for Drought Escape.

Ahmad Muhammad Shafiq MS Wu Bingrui B Wang Huaqi H Kang Dingming D

Plants (Basel, Switzerland) 20200511 5

Terminal drought stress is one of the restrictive factors in rice production and is expected to upsurge under the current situation of climate change. The study evaluated the performance of 2030 rice genotypes under continuous drought stress conditions based on days to flowering (DF). The genotypes under augmented randomized complete block design were sown in May/June of 2017 and 2018 in the field with movable rainout that resulted in huge genetic diversity among the accessions. Descriptive stat ...[more]

PMID: 32403343

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Project description:BACKGROUND AND AIMS:Salt stress leads to attenuated growth and productivity in rice. Transcription factors like heat shock factors (HSFs) represent central regulators of stress adaptation. Heat shock factors of the classes A and B are well established as regulators of thermal and non-thermal stress responses in plants; however, the role of class C HSFs is unknown. Here we characterized the function of the OsHsfC1b (Os01g53220) transcription factor from rice. METHODOLOGY:We analysed the expression of OsHsfC1b in the rice japonica cultivars Dongjin and Nipponbare exposed to salt stress as well as after mannitol, abscisic acid (ABA) and H(2)O(2) treatment. For functional characterization of OsHsfC1b, we analysed the physiological response of a T-DNA insertion line (hsfc1b) and two artificial micro-RNA (amiRNA) knock-down lines to salt, mannitol and ABA treatment. In addition, we quantified the expression of small Heat Shock Protein (sHSP) genes and those related to signalling and ion homeostasis by quantitative real-time polymerase chain reaction in roots exposed to salt. The subcellular localization of OsHsfC1b protein fused to green fluorescent protein (GFP) was determined in Arabidopsis mesophyll cell protoplasts. PRINCIPAL RESULTS:Expression of OsHsfC1b was induced by salt, mannitol and ABA, but not by H(2)O(2). Impaired function of OsHsfC1b in the hsfc1b mutant and the amiRNA lines led to decreased salt and osmotic stress tolerance, increased sensitivity to ABA, and temporal misregulation of salt-responsive genes involved in signalling and ion homeostasis. Furthermore, sHSP genes showed enhanced expression in knock-down plants under salt stress. We observed retarded growth of hsfc1b and knock-down lines in comparison with control plants under non-stress conditions. Transient expression of OsHsfC1b fused to GFP in protoplasts revealed nuclear localization of the transcription factor. CONCLUSIONS:OsHsfC1b plays a role in ABA-mediated salt stress tolerance in rice. Furthermore, OsHsfC1b is involved in the response to osmotic stress and is required for plant growth under non-stress conditions.

Dataset Information

Field Screening of Rice Germplasm (Oryza sativa L. ssp. japonica) Based on Days to Flowering for Drought Escape.

Publications

Field Screening of Rice Germplasm (<i>Oryza sativa</i> L. ssp. <i>japonica</i>) Based on Days to Flowering for Drought Escape.

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