Dataset Information

Patatin-Related Phospholipase pPLAIII? Involved in Osmotic and Salt Tolerance in Arabidopsis.

ABSTRACT: Patatinrelated phospholipases (pPLAs) are acylhydrolyzing enzymes implicated in various processes, including lipid metabolism, signal transduction, plant growth and stress responses, but the function for many specific pPLAs in plants remains unknown. Here we determine the effect of patatinrelated phospholipase A pPLAIII? on Arabidopsis response to abiotic stress. Knockout of pPLAIII? rendered plants more sensitive whereas overexpression of pPLAIII? enhanced plant tolerance to NaCl and drought in seed germination and seedling growth. The pPLAIII?knockout and overexpressing seedlings displayed a lower and higher level of lysolipids and free fatty acids than that of wildtype plants in response to NaCl stress, respectively. These results indicate that pPLAIII? acts a positive regulator of salt and osmatic stress tolerance in Arabidopsis.

SUBMITTER: Li J

PROVIDER: S-EPMC7284883 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Patatin-Related Phospholipase pPLAIIIγ Involved in Osmotic and Salt Tolerance in Arabidopsis.

Li Jianwu J Li Maoyin M Yao Shuaibing S Cai Guangqin G Wang Xuemin X

Plants (Basel, Switzerland) 20200520 5

Patatinrelated phospholipases (pPLAs) are acylhydrolyzing enzymes implicated in various processes, including lipid metabolism, signal transduction, plant growth and stress responses, but the function for many specific pPLAs in plants remains unknown. Here we determine the effect of patatinrelated phospholipase A pPLAIIIγ on Arabidopsis response to abiotic stress. Knockout of pPLAIIIγ rendered plants more sensitive whereas overexpression of pPLAIIIγ enhanced plant tolerance t ...[more]

PMID: 32443904

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Project description:Salinity is one of the major limitations for food production worldwide. Improvement of plant salt-stress tolerance using plant-growth promoting rhizobacteria (PGPR) has arisen as a promising strategy to help overcome this limitation. However, the molecular and biochemical mechanisms controlling PGPR/plant interactions under salt-stress remain unclear. The main objective of this study was to obtain new insights into the mechanisms underlying salt-stress tolerance enhancement in the salt-sensitive Arabidopsis thaliana Col-0 plants, when inoculated with the well-known PGPR strain Burkholderia phytofirmans PsJN. To tackle this, different life history traits, together with the spatiotemporal accumulation patterns for key metabolites and salt-stress related transcripts, were analyzed in inoculated plants under short and long-term salt-stress. Inoculated plants displayed faster recovery and increased tolerance after sustained salt-stress. PsJN treatment accelerated the accumulation of proline and transcription of genes related to abscisic acid signaling (Relative to Dessication, RD29A and RD29B), ROS scavenging (Ascorbate Peroxidase 2), and detoxification (Glyoxalase I 7), and down-regulated the expression of Lipoxygenase 2 (related to jasmonic acid biosynthesis). Among the general transcriptional effects of this bacterium, the expression pattern of important ion-homeostasis related genes was altered after short and long-term stress (Arabidopsis K(+) Transporter 1, High-Affinity K(+) Transporter 1, Sodium Hydrogen Exchanger 2, and Arabidopsis Salt Overly Sensitive 1). In all, the faster and stronger molecular changes induced by the inoculation suggest a PsJN-priming effect, which may explain the observed tolerance after short-term and sustained salt-stress in plants. This study provides novel information about possible mechanisms involved in salt-stress tolerance induced by PGPR in plants, showing that certain changes are maintained over time. This opens up new venues to study these relevant biological associations, as well as new approaches to a better understanding of the spatiotemporal mechanisms involved in stress tolerance in plants.

Dataset Information

Patatin-Related Phospholipase pPLAIII? Involved in Osmotic and Salt Tolerance in Arabidopsis.

Publications

Patatin-Related Phospholipase pPLAIIIγ Involved in Osmotic and Salt Tolerance in <i>Arabidopsis</i>.

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