Development of a TaqMan qPCR assay for detection of Alexandrium spp and application to harmful algal bloom monitoring.
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ABSTRACT: Highlights•An inhouse qPCR assay was developed using the 18s rDNA to detect Alexandrium spp.•qPCR had reduced LOD & improved specificity when compared to light microscopy.•qPCR had a higher correlation to toxicity data when compared to light microscopy•DNA extracts were found to be stable when fixed with Lugol's for >800 days.
SUBMITTER: Hatfield RG
PROVIDER: S-EPMC7285902 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
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