Accelerated evolution of a minimal 63-amino acid dual transcription factor.
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ABSTRACT:
SUBMITTER: Brodel AK
PROVIDER: S-EPMC7286687 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
Accelerated evolution of a minimal 63-amino acid dual transcription factor.
Brödel Andreas K AK Rodrigues Rui R Jaramillo Alfonso A Isalan Mark M
Science advances 20200610 24
Transcription factors control gene expression in all life. This raises the question of what is the smallest protein that can support such activity. In nature, Cro from bacteriophage λ is one of the smallest known repressors (66 amino acids), and activators are typically much larger (e.g., λ cI, 237 amino acids). Previous efforts to engineer a minimal activator from λ Cro resulted in no activity in vivo in cells. In this study, we show that directed evolution results in a new Cro activator-repres ...[more]
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