Dataset Information

Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer.

ABSTRACT: Green alga Chlamydomonas reinhardtii has gained interest as a sustainable resource because it can be easily grown using CO₂ as a carbon source owing to its high CO₂ assimilating activity. Although the robustness of the cell wall of C. reinhardtii makes it difficult to extract its intracellular products, such property is beneficial when using the cell as an ingredient to fabricate "cell-plastic" in this study. The cell layer, which is a component of the cell-plastic, was prepared with an intercellular filler to connect each cell because C. reinhardtii is a single-cell strain. The cell layers were then repeatedly piled to increase the strength of the cell-plastic. To avoid slippage between the cell layers, they were covered with a small amount of a two-dimensional polymer to maintain the flat surface structure of the cell-plastic. Based on the evaluation, the cell-plastic has the potential to be a novel, sustainable plastic using ubiquitous green algal cells in nature.

SUBMITTER: Nakanishi A

PROVIDER: S-EPMC7286992 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer.

Nakanishi Akihito A Iritani Kohei K Sakihama Yuri Y Ozawa Nanami N Mochizuki Ayano A Watanabe Marina M

AMB Express 20200610 1

Green alga Chlamydomonas reinhardtii has gained interest as a sustainable resource because it can be easily grown using CO<sub>2</sub> as a carbon source owing to its high CO<sub>2</sub> assimilating activity. Although the robustness of the cell wall of C. reinhardtii makes it difficult to extract its intracellular products, such property is beneficial when using the cell as an ingredient to fabricate "cell-plastic" in this study. The cell layer, which is a component of the cell-plastic, was pre ...[more]

PMID: 32524300

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Project description:BACKGROUND AND AIMS: Under stress-promoting conditions unicellular algae can undergo programmed cell death (PCD) but the mechanisms of algal cellular suicide are still poorly understood. In this work, the involvement of caspase-like proteases, DNA cleavage and the morphological occurrence of cell death in wasp venom mastoparan (MP)-treated Chlamydomonas reinhardtii were studied. METHODS: Algal cells were exposed to MP and cell death was analysed over time. Specific caspase inhibitors were employed to elucidate the possible role of caspase-like proteases. YVADase activity (presumably a vacuolar processing enzyme) was assayed by using a fluorogenic caspase-1 substrate. DNA breakdown was evaluated by DNA laddering and Comet analysis. Cellular morphology was examined by confocal laser scanning microscopy. KEY RESULTS: MP-treated C. reinhardtii cells expressed several features of necrosis (protoplast shrinkage) and vacuolar cell death (lytic vesicles, vacuolization, empty cell-walled corpse-containing remains of digested protoplast) sometimes within one single cell and in different individual cells. Nucleus compaction and DNA fragmentation were detected. YVADase activity was rapidly stimulated in response to MP but the early cell death was not inhibited by caspase inhibitors. At later time points, however, the caspase inhibitors were effective in cell-death suppression. Conditioned medium from MP-treated cells offered protection against MP-induced cell death. CONCLUSIONS: In C. reinhardtii MP triggered PCD of atypical phenotype comprising features of vacuolar and necrotic cell deaths, reminiscent of the modality of hypersensitive response. It was assumed that depending on the physiological state and sensitivity of the cells to MP, the early cell-death phase might be not mediated by caspase-like enzymes, whereas later cell death may involve caspase-like-dependent proteolysis. The findings substantiate the hypothesis that, depending on the mode of induction and sensitivity of the cells, algal PCD may take different forms and proceed through different pathways.

Dataset Information

Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer.

Publications

Construction of cell-plastics as neo-plastics consisted of cell-layer provided green alga Chlamydomonas reinhardtii covered by two-dimensional polymer.

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