Rapid and specific detection of oxidized LDL/?2GPI complexes via facile lateral flow immunoassay.

ABSTRACT: ?2-Glycoprotein I (?2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/?2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both systemic and non-systemic autoimmune diseases. Hence, serological level of oxLDL/?2GPI complexes may represent one crucial clinical parameter for disease prognosis of atherosclerosis-related diseases. Herein, we established a simple, specific and rapid gold nanoparticle (GNP) based lateral flow immunoassay (LFIA) to quantify oxLDL/?2GPI complexes from test samples. Specificities of hybridoma cell-derived monoclonal antibodies against antigen, optimal conditions for conjugation of antibody with GNP, and sensitivity of oxLDL/?2GPI LFIA in comparison to an ELISA-based detection method were assessed accordingly. The established oxLDL/?2GPI LFIA was capable of detecting oxLDL/?2GPI specifically without interference from autoantibodies and solitary components of oxLDL/?2GPI present in test samples. A significant correlation (R2 > 0.8) was also obtained with the oxLDL/?2GPI LFIA when compared to the ELISA-based detection. On the whole, the oxLDL/?2GPI LFIA remains advantageous over the oxLDL/?2GPI ELISA. The unnecessary washing step, short developmental and analytical time support facile and rapid detection of oxLDL/?2GPI as opposed to the laborious ELISA system.

SUBMITTER: Tan XW 

PROVIDER: S-EPMC7287255 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature