Dataset Information

Phytochemical andrographolide modulates NF-?B and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease.

ABSTRACT: Andrographis paniculata has been an important plant for traditional medicine in Asia for centuries. Andrographolide is the primary bioactive phytochemical from the plant and is known to exhibit many different protective effects through modulation of various proteins and signaling pathways. Andrographolide has been reported to exert anti-inflammatory and neuroprotective effects as well as being an antioxidant itself. We therefore studied whether andrographolide could provide protective effects to the SH-SY5Y neuroblastoma cell model for Parkinson's disease. In this study, we observed andrographolide inhibiting activation of NF-?B p65 (nuclear factor kappa-light-chain-enhancer of activated B cells) and JNK MAPK (c-Jun N-terminal Kinase Mitogen-Activated Protein Kinase) pathways, however, it did not provide any protective effect against induced stress in the SH-SY5Y cells. We propose the sustained low-level activation of JNK and the inhibition of NF-?B promoted ROS (Reactive Oxygen Species) production that yielded the observed cell death. Therefore, the protective effects observed with andrographolide appear to be cell/tissue specific responses.

SUBMITTER: Ketterman AJ

PROVIDER: S-EPMC7287258 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease.

Ketterman Albert J AJ Wongtrakul Jeerang J Saisawang Chonticha C

Heliyon 20200609 6

<i>Andrographis paniculata</i> has been an important plant for traditional medicine in Asia for centuries. Andrographolide is the primary bioactive phytochemical from the plant and is known to exhibit many different protective effects through modulation of various proteins and signaling pathways. Andrographolide has been reported to exert anti-inflammatory and neuroprotective effects as well as being an antioxidant itself. We therefore studied whether andrographolide could provide protective eff ...[more]

PMID: 32551381

Similar Datasets

Project description:BackgroundMaleic acid is a multi-functional chemical widely used in the field of industrial chemistry for producing food additives and food contact materials. As maleic acid may contaminate food by the release from food packages or intentional addition, it raises the concern about the effects of excessive dietary exposure to maleic acid on human health. However, the influence of maleic acid on human health has not been thoroughly studied. In silico toxicogenomics approaches have found the association between maleic acid and nervous system disease in human. The aim of this study is to experimentally explore the effects of maleic acid on human neuronal cells.MethodsA microarray-based transcriptome profiling was performed to offer a better understanding of the effects of maleic acid on human health. Gene expression profiles of human neuroblastoma SH-SY5Y cells exposed to three concentrations of maleic acid (10, 50, and 100 μM) for 24 h were analyzed. Genes which were differentially expressed in dose-dependent manners were identified and further analyzed with an enrichment analysis. The expression profile of selected genes related to the inferred functional changes was validated using quantitative polymerase chain reaction (qPCR). Specific fluorescence probes were applied to observe the inferred functional changes in maleic acid-treated neuronal cells.ResultsA total of 316 differentially expressed genes (141 upregulated and 175 downregulated) were identified in response to the treatment of maleic acid. The enrichment analysis showed that DNA binding and metal ion binding were the significant molecular functions (MFs) of the neuronal cells affected by maleic acid. Maleic acid exposure decreased the expression of genes associated with calcium and thiol levels of the cells in a dose-dependent manner. The levels of intracellular calcium and thiol levels were also affected by maleic acid dose-dependent.DiscussionThe exposure to maleic acid is found to decrease the cellular calcium and thiol levels in human neuronal cells at both transcriptional and functional levels. This study reported the first transcriptomic profiling of human neuronal cells treated with maleic acid. It is also the first experimental validation of chemical effects predicted by in silico toxicogenomics approaches. The proposed approach may be useful in understanding the potential effects of other poorly characterized chemicals on human health.

Dataset Information

Phytochemical andrographolide modulates NF-?B and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease.

Publications

Phytochemical andrographolide modulates NF-κB and JNK in human neuroblastoma SH-SY5Y cells, a cell model for Parkinson's disease.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets