Dataset Information

Tyrosinase Inhibition and Kinetic Details of Puerol A Having But-2-Enolide Structure from Amorpha fruticosa.

ABSTRACT: Puerol A (1) from Amorpha fruticosa showed highly potent inhibition against both monophenolase (IC₅₀ = 2.2 ?M) and diphenolase (IC₅₀ = 3.8 ?M) of tyrosinase. We tried to obtain a full story of enzyme inhibitory behavior for inhibitor 1 because the butenolide skeleton has never been reported as a tyrosinase inhibitor. Puerol A was proved as a reversible, competitive, simple slow-binding inhibitor, according to the respective parameters; k₃ = 0.0279 ?M^-1 min^-1 and k₄ = 0.003 min^-1. A longer lag-phase and a reduced static-state activity of the enzyme explained that puerol A had a tight formation of the complex with E_met. Dose-dependent inhibition was also confirmed by high-performance liquid chromatography (HPLC) analysis using N-acetyl-l-tyrosine as a substrate, which was completely inhibited at 20 ?M. A high binding affinity of 1 to tyrosinase was confirmed by fluorescence quenching analysis. Moreover, puerol A decreased melanin content in the B16 melanoma cell dose-dependently with an IC₅₀ of 11.4 ?M.

SUBMITTER: Kim JH

PROVIDER: S-EPMC7287670 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Tyrosinase Inhibition and Kinetic Details of Puerol A Having But-2-Enolide Structure from Amorpha fruticosa.

Kim Jeong Ho JH Jang Da Hyun DH Lee Ki Won KW Kim Kwang Dong KD Shah Abdul Bari AB Zhumanova Kamila K Park Ki Hun KH

Molecules (Basel, Switzerland) 20200518 10

Puerol A (1) from Amorpha fruticosa showed highly potent inhibition against both monophenolase (IC50 = 2.2 μM) and diphenolase (IC50 = 3.8 μM) of tyrosinase. We tried to obtain a full story of enzyme inhibitory behavior for inhibitor 1 because the butenolide skeleton has never been reported as a tyrosinase inhibitor. Puerol A was proved as a reversible, competitive, simple slow-binding inhibitor, according to the respective parameters; k3</s ...[more]

PMID: 32443441

Similar Datasets

Project description:BackgroundWater availability and nutrient-status of soils play crucial roles in seedling establishment and plant survival in coal-spoiled areas worldwide. Restoration of spoils pertains to the application of proper doses of nutrients and water, and selection of particular plant species for efficient revegetation. This study aimed at examining the potential effects of different combinations of soil-water and fertilizers (nitrogen, N and phosphorus, P) on morpho-physiological and biochemical attributes of Amorpha fruticosa grown in coal-mined spoils. Three factors five-level central-composite-design with optimization technique response surface methodology (rsm) was used to optimize water irrigation and fertilizer application strategies.ResultsOur results revealed a strong correlation between experimental data and predicted values developed from the rsm model. The best responses of A. fruticosa in terms of plant height, stem diameter, root length, and dry biomass were observed under a high-water regime. Low-water regime caused a notable reduction in growth-associated parameters, and fertilization with either N or P did not show positive effects on those parameters, indicating that soil-water was the most influential factor for growth performance. Leaf water potential, gas-exchange parameters, and chlorophyll content significantly increased under high levels of soil-water, N and P, suggesting a synergistic effect of these factors for the improvement of photosynthesis-related parameters. At low soil-water contents and N-P fertilizer application levels, enhanced accumulation of malondialdehyde and proline indicated that A. fruticosa suffered from oxidative and osmotic stresses. Amorpha fruticosa also responded to oxidative stress by accelerating the activities of superoxide dismutase, catalase, and peroxidase. The effects of both fertilizers relied on soil-water, and fertilization was most effective under well-watered conditions. The maximum growth of A. fruticosa was observed under the combination of soil-water, N-dose and P-dose at 76% field capacity, 52.0?mg?kg-?1 and 49.0?mg?kg-?1, respectively.ConclusionOur results demonstrate that rsm effectively designed appropriate doses of water and N-P fertilizer to restore coal-spoiled soils. Furthermore, A. fruticosa responded to low-water and fertilizer-shortage by upregulating defensive mechanism to avoid damage induced by such deficiencies. Finally, our findings provide effective strategies for revegetation of coal-contaminated spoils with A. fruticosa using appropriate doses of water and N-P fertilizers.

Dataset Information

Tyrosinase Inhibition and Kinetic Details of Puerol A Having But-2-Enolide Structure from Amorpha fruticosa.

Publications

Tyrosinase Inhibition and Kinetic Details of Puerol A Having But-2-Enolide Structure from <i>Amorpha fruticosa</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure