Age Mosaicism across Multiple Scales in Adult Tissues.
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ABSTRACT:
SUBMITTER: Arrojo E Drigo R
PROVIDER: S-EPMC7289515 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Publications
Age Mosaicism across Multiple Scales in Adult Tissues.
Arrojo E Drigo Rafael R Lev-Ram Varda V Tyagi Swati S Ramachandra Ranjan R Deerinck Thomas T Bushong Eric E Phan Sebastien S Orphan Victoria V Lechene Claude C Ellisman Mark H MH Hetzer Martin W MW
Cell metabolism 20190606 2
Most neurons are not replaced during an animal's lifetime. This nondividing state is characterized by extreme longevity and age-dependent decline of key regulatory proteins. To study the lifespans of cells and proteins in adult tissues, we combined isotope labeling of mice with a hybrid imaging method (MIMS-EM). Using <sup>15</sup>N mapping, we show that liver and pancreas are composed of cells with vastly different ages, many as old as the animal. Strikingly, we also found that a subset of fibr ...[more]
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