Dataset Information

Enhanced yield performance of transgenic cry1C* rice in saline-alkaline soil.

ABSTRACT: China has a large area of saline-alkaline land that can be utilized for the cultivation of transgenic rice. Therefore, the growth and reproductive behavior of transgenic rice are not only a problem for production that needs to be resolved, but also an important aspect of environmental risk assessment for saline alkali soil. In the present study, an insect-resistant transgenic cry1C* rice, T1C-19, was grown in farmland and saline-alkaline soils. The transcription and translation of the exogenous cry1C*, and vegetative and reproductive fitness, such as plant height, tiller number, biomass, filled grain number and weight per plant, were assessed. Our findings indicated that the transcription and translation of exogenous cry1C* gene in T1C-19 rice grown in saline-alkaline soil were lower than that grown in farmland; however, the correlation was not significant. The vegetative and reproductive growth abilities of T1C-19 were lower than that of the parental rice, Minghui63 (MH63), in farmland. In alkaline-saline soil, except for tiller number and biomass, there were no significant differences between T1C-19 and MH63 in other vegetative indices. In contrast, the reproductive indices of T1C-19 were significantly higher than those of MH63. The results suggested that T1C-19 had a strong reproductive capacity, and significantly reduced the loss of yield caused by insects, thereby leading to a higher yield than that of MH63 grown in saline-alkaline soils. This may promote the cultivation of saline-alkaline soil to permit farming of T1C-19 in China in the future, despite the possible increased ecological risks.

SUBMITTER: Fu J

PROVIDER: S-EPMC7289519 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:BackgroundZinc finger proteins (ZFPs) play an important role in regulating plant responses to abiotic stress. However, little is known about the function of LSD1-like-type ZFP in saline-alkaline (SA) stress resistance of rice. In this study, OsLOL5 (GenBank No. AJ620677), containing two LSD1-like-type C2C2 domains, was isolated and analyzed its protection roles in transgenic plants and yeast. OsLOL5 was located in the nucleus as evidenced by the bombardment of onion epidermal cells.ResultsOsLOL5 expression significantly increased in rice leaves and roots under 150 mmol L-1 NaCl, 30 mM NaHCO3, and 10 mmol L-1 H2O2 treatment, respectively. Overexpression of OsLOL5 in yeast resulted in SA tolerance at significant level. Transgenic Arabidopsis plants overexpressing OsLOL5 grew well in the presence of both NaCl and NaHCO3 treatments, whereas wild-type plants exhibited chlorosis, stunted growth phenotype, and even death. SA stress caused significant changes in the malondialdehyde (MDA) contents in non-transgenic plants compared with those in transgenic lines. Transgenic rice overexpressing OsLOL5 exhibited stronger resistance than NT under NaHCO3 treatment, as demonstrated by its greater shoot length, and fresh weight. The genes associated with oxidative stress, such as OsAPX2, OsCAT, OsCu/Zn-SOD, and OsRGRC2, were significantly upregulated in OsLOL5-overexpressing rice. The results suggested that OsLOL5 improved SA tolerance in plants, and regulated oxidative and salinity stress retardation via the active oxygen detoxification pathway.ConclusionsThe yeast INVScI bacterium grew significantly better than the control strain under NaCl, NaHCO3, and H2O2 treatments. These findings illustrated that OsLOL5 overexpression enhanced yeast resistance for SA stress through active oxygen species. The present study showed that the OsLOL5 genes involved in the ROS signaling pathways may combine with the model plant Arabidopsis and rice in LDS1-type ZFP by ROS signaling pathways that regulate cell necrosis. We speculated that the OsLOL5 active oxygen scavenging system may have coordinating roles. The present study further revealed that OsLOL5 ZFP could regulate oxidative stress function, but could also provide a basis for salt-resistant rice strains.

Project description:Genetic modification of Cry-proteins from Bacillus thuringiensis (Bt) is a common practice in economically important crops to improve insecticide resistance and reduce the use of pesticides. However, introduction of these genes can have unintended side effects, which should be closely monitored for effective breeding and crop management. To determine the potential cause of these negative effects, we explored assimilate partitioning in the transgenic Bt rice line T1c-19 (Cry1C*), which was compared with that of its wild-type counterpart Minghui 63 (MH63) under different potassium fertilization application treatment conditions. In a pot experiment, 0, 0.4, and 0.6 g K2O was applied per kg of dry soil to determine the phloem transport characteristics of the two rice lines. We used a variety of assessment indicators ranging from morphological to physiological aspects, including the number of large and small vascular bundles in the neck internode at the heading stage, the diameter and bleeding intensity of the neck internode at the filling stage, and the content and apparent ratio of transferred non-structural carbohydrates (NSC) in the culm and sheath from the heading to maturing stages. The K utilization and grain yield at the maturing stage were also concerned. Results presented that the mean setting rate and grain yield of T1c-19 (Cry1C*) decreased by 22.3% and 26.2% compared to those in MH63, respectively. Compared to MH63, the K concentration and accumulation were significantly higher in the culms and leaves, but significantly lower in grain of T1c-19 (Cry1C*). T1c-19 (Cry1C*) had less apparent NSC efflux in the culm and sheath, fewer small vascular bundles, and a smaller diameter and bleeding intensity of the neck internode than MH63. In addition, linear correlation analysis indicated that there were positive correlations among grain yield, setting rate, the apparent NSC efflux in the culm and sheath, number of small vascular bundles, and the neck internode diameter and bleeding intensity. These unintended effects may directly or indirectly be caused by insertion of exogenous Bt (Cry1C*) gene, which should be further considered in the future breeding of transgenic crops.

Dataset Information

Enhanced yield performance of transgenic cry1C* rice in saline-alkaline soil.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets