Ontology highlight
ABSTRACT:
SUBMITTER: Zhang L
PROVIDER: S-EPMC7289597 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Zhang Li L Takahashi Yohei Y Hsu Po-Kai PK Kollist Hannes H Merilo Ebe E Krysan Patrick J PJ Schroeder Julian I JI
eLife 20200528
Sucrose-non-fermenting-1-related protein kinase-2s (SnRK2s) are critical for plant abiotic stress responses, including abscisic acid (ABA) signaling. Here, we develop a genetically encoded reporter for SnRK2 kinase activity. This sensor, named SNACS, shows an increase in the ratio of yellow to cyan fluorescence emission by OST1/SnRK2.6-mediated phosphorylation of a defined serine residue in SNACS. ABA rapidly increases FRET efficiency in <i>N. benthamiana</i> leaf cells and <i>Arabidopsis</i> gu ...[more]