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Characterization of lymphocyte subsets in ascitic fluid and peripheral blood of decompensated cirrhotic patients with chronic hepatitis C and alcoholic liver disease: A pivotal study.

ABSTRACT: Hepatitis C virus and alcoholic liver disease are major causes of chronic liver diseases worldwide. Little is known about differences between chronic hepatitis C and alcoholic liver disease in terms of lymphocytes' sub-population. Aim of the present study was to compare the sub-populations of lymphocytes in both ascitic compartment and peripheral blood in patients with decompensated liver cirrhosis due to chronic hepatitis C and alcoholic liver disease. Patients with decompensated liver cirrhosis due to hepatitis C virus or alcoholic liver disease evaluated from April 2014 to October 2016 were enrolled. Whole blood and ascitic fluid samples were stained with monoclonal antibodies specific for human TCR??, TCR??, CD3, CD4, CD8, CD19, CCR6, CD16, CD56, CD25, HLA-DR, V?24. Sixteen patients with decompensated liver cirrhosis were recruited (9 with hepatitis C virus and 7 with alcoholic liver disease). In ascitic fluid, the percentage of both CD3⁺CD56^- and CD3⁺CD56⁺iNKT cells resulted higher in hepatitis C virus patients than in alcoholic liver disease patients (1.82?±?0.35% vs 0.70?±?0.42% (p?+CD56^- and CD3⁺CD56⁺iNKT cells resulted significantly higher in alcoholic liver disease than in hepatitis C virus patients (4.70?±?2.69% vs 1.50?±?1.21% (p?+CD4⁺ cells and a higher percentage of CD3^-CD16⁺, V?24⁺CD3⁺CD56^- and V?24⁺CD3⁺CD56⁺iNKT cells in ascitic fluid than hepatitis C virus positive subjects. Further studies might analyze the role of immune cells in the vulnerability toward infections and detect potential targets for new treatments especially for alcoholic liver disease patients.

SUBMITTER: Romanelli RG

PROVIDER: S-EPMC7290250 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature

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Characterization of lymphocyte subsets in ascitic fluid and peripheral blood of decompensated cirrhotic patients with chronic hepatitis C and alcoholic liver disease: A pivotal study.

Romanelli Roberto Giulio RG Vitiello Gianfranco G Gitto Stefano S Giudizi Maria Grazia MG Biagiotti Roberta R Carraresi Alessia A Vizzutti Francesco F Laffi Giacomo G Almerigogna Fabio F

International journal of immunopathology and pharmacology 20200101

Hepatitis C virus and alcoholic liver disease are major causes of chronic liver diseases worldwide. Little is known about differences between chronic hepatitis C and alcoholic liver disease in terms of lymphocytes' sub-population. Aim of the present study was to compare the sub-populations of lymphocytes in both ascitic compartment and peripheral blood in patients with decompensated liver cirrhosis due to chronic hepatitis C and alcoholic liver disease. Patients with decompensated liver cirrhosi ...[more]

PMID: 32524881

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Dataset Information

Characterization of lymphocyte subsets in ascitic fluid and peripheral blood of decompensated cirrhotic patients with chronic hepatitis C and alcoholic liver disease: A pivotal study.

Publications

Characterization of lymphocyte subsets in ascitic fluid and peripheral blood of decompensated cirrhotic patients with chronic hepatitis C and alcoholic liver disease: A pivotal study.

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