Dataset Information

Validation and quantification of major biomarkers in 'Mahasudarshan Churna'- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography.

ABSTRACT:

Background

Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever (especially chronic type), cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections.

Methods

Validation and quantification of oleanolic acid (OA), ursolic acid (UA), mangiferin (M), gallic acid (GA), quercetin (Q) and curcumin (C) in commercial MC formulations by HPTLC method. Mobile phase, hexane: ethyl acetate: acetone (16.4: 3.6: 0.2, v/v) was used for the separation of OA and UA; ethyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2?v/v) for the development of M; and toluene: ethyl acetate: formic acid (13.5: 9: 0.6?v/v) for the separation of GA, Q and C in crude sample extracts. Visualization and scanning were performed at ??=?530?nm for OA and UA, at ??=?254?nm for M and at ??=?366?nm for GA, Q and C. In addition, HPLC-PDA analysis was used to confirm the HPTLC results.

Results

Major bio-active compounds in MC formulations were oleanolic acid (1.54-1.78%), mangiferin (1.38-1.52%) and gallic acid (1.01-1.15%); followed by ursolic acid (0.79-0.98%), curcumin (0.45-0.67%) and quercetin (0.22-0.34%).

Conclusion

Analysis of bio-active compounds in the present study was performed using HPTLC methods and later HPTLC results were compared with HPLC. These two methods give comparable results and there was no statistically significant difference between the mean values for all extracts. Present study concluded that this HPTLC technique is low cost, fast, precise, and accurate which can be employed for the quantification of xanthonoid (M), triterpenoids (OA, UA) and phenolics (GA, Q and C) in samples/formulations. Furthermore, present HPTLC method can be conveniently employed for routine quality control analysis of all the six marker compounds in marketed Ayurvedic/herbal formulations.

SUBMITTER: Kaur P

PROVIDER: S-EPMC7291524 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Publications

Validation and quantification of major biomarkers in 'Mahasudarshan Churna'- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography.

Kaur Prabhjot P Gupta R C RC Dey Abhijit A Malik Tabarak T Pandey Devendra Kumar DK Pandey Devendra Kumar DK

BMC complementary medicine and therapies 20200611 1

<h4>Background</h4>Mahasudarshan Churna (MC) is a polyherbal Ayurvedic medicine that is employed in fever (especially chronic type), cold and malaria, improvement of digestion and appetite, removes toxins from the blood, boosts immunity and protects against common bacterial infections.<h4>Methods</h4>Validation and quantification of oleanolic acid (OA), ursolic acid (UA), mangiferin (M), gallic acid (GA), quercetin (Q) and curcumin (C) in commercial MC formulations by HPTLC method. Mobile phase, ...[more]

PMID: 32527318

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Project description:BackgroundNayopayam kwatha (NK) is a well-known polyherbal formulation widely used to cure respiratory ailments, heart problems, and postnatal difficulties. Literature suggests that so far no standardization protocol was developed for NK to validate its quality and purity.Objective(s)To develop a standardization protocol for NK based on the marker phytoconstituents present in the individual herbs of the formulation.Materials and methodsThe roots of bala [Sida cordifolia (B1) and Sida retusa (B2)], seeds of jeeraka (Cuminum cyminum), and rhizomes of nagara (Zingiber officinale) were the ingredients of NK. Since there were two source plants for bala, two sets of NK (NKB1 and NKB2) were prepared in the ratio 3:2:1 as per Vaidya Manorama and 10:1:1 as per Arogyaraksha Kalpadruma along with 1:1:1 as per the general way of Ayurvedic polyherbal decoctions. Both the individual herbs and the kwatha (decoction) prepared were analyzed in terms of pharmacognostical, organoleptic, and physcicochemical parameters as per the standard methods. Phytochemical analysis of the individual herbs resulted in the isolation of major phytoconstituents and the kwatha was quantified in terms of marker compounds with the aid of HPLC.ResultsHPLC quantification suggests that appreciable amount of marker phytoconstituents of individual herbs are present in the kwatha. Thus, the isolated compounds luteolin (C. cyminum), 6-gingerol (Z. officinale), β-sitosterol (S. retusa), and ecdysterone (S.cordifolia) can be used as markers to standardize NK.ConclusionCharacteristics of NK, as well as its individual drugs, were well-established. The present study of NK with respect to its phytochemistry revealed that the classical drug ratios of the polyherbal formulation are of utmost importance rather than the ingredients in equal proportion. The characterization parameters of individual herbs and kwatha described in this study may serve as a standard reference for quality control analysis of NK and the method developed in this study can be used as a reliable technique for standardization of NK to ensure the purity and quality of raw drugs used.

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Dataset Information

Validation and quantification of major biomarkers in 'Mahasudarshan Churna'- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography.

Background

Methods

Results

Conclusion

Publications

Validation and quantification of major biomarkers in 'Mahasudarshan Churna'- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography.

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