Ontology highlight
ABSTRACT:
Methods: We recorded excitatory postsynaptic potential in layer V pyramidal neurons in the PFC by intracellular recording method to assess synaptic plasticity in the form of long-term potentiation (LTP). We also performed N-methyl-d-aspartate acid (NMDA) receptor binding assay in the PFC and hippocampus using radiolabeled NMDA receptor antagonist [3 H]MK-801.
Results: Theta-burst stimulation induced LTP in the PFC of genetic control, WKY/Ezo, whereas failed to induce LTP in that of SHRSP/Ezo. The Kd value of [3 H]MK-801 binding for NMDA receptors in the PFC of SHRSP/Ezo was higher than in the WKY/Ezo. Neither the Bmax nor Kd of [3 H]MK-801 binding in the SHRSP/Ezo hippocampus was significantly different to WKY/Ezo.
Conclusion: These results suggest that the AD/HD animal model SHRSP/Ezo has NMDA receptor dysfunction in the PFC.
SUBMITTER: Shikanai H
PROVIDER: S-EPMC7292284 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Shikanai Hiroki H Oshima Nobuhiro N Kawashima Hidekazu H Kimura Shin-Ichi SI Hiraide Sachiko S Togashi Hiroko H Iizuka Kenji K Ohkura Kazue K Izumi Takeshi T
Neuropsychopharmacology reports 20180226 2
<h4>Aim</h4>We previously reported that stroke-prone spontaneously hypertensive rat/Ezo (SHRSP/Ezo) has high validity as an attention deficit/hyperactivity disorder (AD/HD) animal model, based on its behavioral phenotypes, such as inattention, hyperactivity, and impulsivity. Fronto-cortical dysfunction is implicated in the pathogenesis of AD/HD. In this study, we investigated prefrontal cortex (PFC) function in SHRSP/Ezo rats by electrophysiological methods and radioreceptor assay.<h4>Methods</h ...[more]