Dataset Information

Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses.

ABSTRACT: rgsCaM has been reported as a calmodulin-like (CML) factor induced by viral infection in Nicotiana. There are three CMLs that belong to the rgsCaM family in Arabidopsis thaliana. In this study, we found a total of 5 NbrgsCaM coding sequences in N. benthamiana genome. We analyzed transcription patterns of NbrgsCaMs in transgenic plants expressing a ?-glucuronidase (GUS) under the promoter of NbrgsCaMs by histochemistry staining and RT-qPCR. Similar to their Arabidopsis homologs, most NbrgsCaMs have an overlapping but distinct expression pattern in response to developmental and environmental changes. Specifically, the NbrgsCaM4 promoter exhibited robust activity and showed distinct regulatory response to viral infection, developmental stages and other abiotic stimuli. Overall, these findings provide clues for further understanding of the NbrgsCaM family genes in regulating plant growth and development under biotic stress and environmental stimulation.

SUBMITTER: Liu D

PROVIDER: S-EPMC7296017 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses.

Liu Dandan D Yang Qiuying Q

Scientific reports 20200615 1

rgsCaM has been reported as a calmodulin-like (CML) factor induced by viral infection in Nicotiana. There are three CMLs that belong to the rgsCaM family in Arabidopsis thaliana. In this study, we found a total of 5 NbrgsCaM coding sequences in N. benthamiana genome. We analyzed transcription patterns of NbrgsCaMs in transgenic plants expressing a β-glucuronidase (GUS) under the promoter of NbrgsCaMs by histochemistry staining and RT-qPCR. Similar to their Arabidopsis homologs, most NbrgsCaMs ha ...[more]

PMID: 32541846

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Project description:BackgroundPlant fatty acid alpha-dioxygenases (alpha-DOX) are oxylipin-forming enzymes induced by biotic and abiotic stresses, which also participate in developmental processes. In Nicotiana attenuata, herbivory strongly induces the expression of an alpha-dox1 gene. To determine its role, we silenced its expression using Agrobacterium-mediated plant transformation with an inverted repeat construct. More than half of the transformed lines showed a severe dwarf growth phenotype that was very similar to the phenotype of tomato plants mutated at a second alpha-dox isoform. This led us to identify the corresponding alpha-dox2 gene in N. attenuata and examine the regulation of both alpha-dox genes as well as the consequences of their silencing in plant development and anti-herbivore defense.ResultsThe transformed lines exhibiting a dwarf growth phenotype are co-silenced for both alpha-dox genes resulting in a nearly complete suppression of alpha-DOX activity, which is associated with increases in ABA, JA and anthocyanin levels, all metabolic signatures of oxidative stress. The other lines, only silenced for alpha-dox1, developed similarly to wild-type plants, exhibited a 40% reduction of alpha-DOX activity resulting in a 50% reduction of its main product in planta (2-HOT) and showed no signs of oxidative stress. In contrast to alpha-dox1, the expression of alpha-dox2 gene is not induced by wounding or elicitors in the oral secretions of Manduca sexta. Instead, alpha-dox2 is expressed in roots and flowers which lack alpha-dox1 expression, but both genes are equally regulated during leaf maturation. We transiently silenced alpha-dox gene copies with gene-specific constructs using virus induced gene silencing and determined the consequences for plant development and phytohormone and 2-HOT levels. While individual silencing of alpha-dox1 or alpha-dox2 had no effects on plant growth, the co-suppression of both alpha-dox genes decreased plant growth. Plants transiently silenced for both alpha-dox genes had increased constitutive levels of JA and ABA but silencing alpha-dox1 alone resulted in lower M. sexta-induced levels of JA, 2-HOT and ABA.ConclusionsThus, both alpha-dox isoforms function in the development of N. attenuata. In leaf maturation, the two alpha-dox genes have overlapping functions, but only alpha-dox2 is involved in root and flower development and only alpha-dox1 functions in anti-herbivore defense.

Dataset Information

Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses.

Publications

Expression patterns of NbrgsCaM family genes in Nicotiana benthamiana and their potential roles in development and stress responses.

Similar Datasets

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