SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds.
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ABSTRACT:
SUBMITTER: Li C
PROVIDER: S-EPMC7296638 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds.
Li Chao C Zong Yuan Y Jin Shuai S Zhu Haocheng H Lin Dexing D Li Shengnan S Qiu Jin-Long JL Wang Yanpeng Y Gao Caixia C
Genome biology 20200616 1
We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase target sites to generate multiplexed base editing. By using paired sgRNAs, SWISS can produce insertions/deletions in addition to base editing. Rice mutants are generated using the SWISS system with efficiencies of cytosine conversion of 25.5%, aden ...[more]
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