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Understanding the Nano-Bio Interactions and the Corresponding Biological Responses.

ABSTRACT: Due to the increasing amount of work being put into the development of nanotechnology, the field of nanomaterials holds great promise for revolutionizing biomedicine. However, insufficient understanding of nanomaterial-biological microenvironment (nano-bio) interactions hinders the clinical translation of nanomedicine. Therefore, a systematic understanding of nano-bio interaction is needed for the intelligent design of safe and effective nanomaterials for biomedical applications. In this review, we summarize the latest experimental and theoretical developments in the fields of nano-bio interfaces and corresponding biological outcomes from the perspective of corona and redox reactions. We also show that nano-bio interaction can offer a variety of multifunctional platforms with a broad range of applications in the field of biomedicine. The potential challenges and opportunities in the study of nano-bio interfaces are also provided.

SUBMITTER: Tian X

PROVIDER: S-EPMC7298559 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Understanding the Nano-Bio Interactions and the Corresponding Biological Responses.

Tian Xin X Chong Yu Y Ge Cuicui C

Frontiers in chemistry 20200610

Due to the increasing amount of work being put into the development of nanotechnology, the field of nanomaterials holds great promise for revolutionizing biomedicine. However, insufficient understanding of nanomaterial-biological microenvironment (nano-bio) interactions hinders the clinical translation of nanomedicine. Therefore, a systematic understanding of nano-bio interaction is needed for the intelligent design of safe and effective nanomaterials for biomedical applications. In this review, ...[more]

PMID: 32587847

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Project description:The merits of nanomedicines are significantly impacted by the surrounding biological environment. Similar to the protein corona generated on the surface of nanoparticles in the circulation system, the intracellular protein corona (IPC) might be formed on nanoparticles when transported inside the cells. However, little is known currently about the formation of IPC and its possible biological influence. Methods: Caco-2 cells, a classical epithelial cell line, were cultured in Transwell plates to form a monolayer. Gold nanoparticles (AuNPs) were prepared as the model nanomedicine due to their excellent stability. Here we focused on identifying IPC formed on the surface of AuNPs during cell transport. The nanoparticles in the basolateral side of the Caco-2 monolayer were collected and analyzed by multiple techniques to verify IPC formation. High-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics was utilized to analyze the composition of IPC proteins. In particular, we established a dual-filtration strategy to exclude various interference in IPC identification. Based on the subcellular localization of specific IPC proteins, we elicited the nano-trafficking network of AuNPs. The transport pathways of AuNPs identified by proteomic analysis were also verified by various conventional technologies. Finally, we explored the influence of IPC on the uptake and stress response of endothelium. Results: The existence of IPC was demonstrated on the surface of AuNPs, in which 227 proteins were identified. Among them, 40 proteins were finally ascertained as the specific IPC proteins. The subcellular location analysis indicated that these "specific" IPC proteins could back-track the transport pathways of nanoparticles in the epithelial cell monolayer. According to the subcellular distribution of IPC proteins and co-localization, we discovered a new pathway of nanoparticles from endosomes to secretory vesicles which was dominant during the transcytosis. After employing conventional imageology and pharmacology strategies to verify the result of proteomic analysis, we mapped a comprehensive intracellular transport network. Our study also revealed the merits of IPC analysis, which could readily elucidate the molecular mechanisms of transcytosis. Besides, the IPC proteins increased the uptake and stress response of endothelium, which was likely mediated by extracellular matrix and mitochondrion-related IPC proteins. Conclusion: The comprehensive proteomic analysis of IPC enabled tracing of transport pathways in epithelial cells as well as revealing the biological impact of nanoparticles on endothelium.

Dataset Information

Understanding the Nano-Bio Interactions and the Corresponding Biological Responses.

Publications

Understanding the Nano-Bio Interactions and the Corresponding Biological Responses.

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