Marked variability in bioactivity between commercially available bovine colostrum for human use; implications for clinical trials.

ABSTRACT:

Background

Colostrum, the milk produced during first few days after birth, is rich in immunoglobulins, antimicrobial peptides & growth factors. Multiple clinical trials using bovine colostrum are ongoing but with no assessment of test product bioactivity.

Objectives

To examine variability of bioactivity between 20 commercial colostrum products, contribution of TGFβ and EGFR in mediating effects, heat sensitivity of bioactivity and changes in bioactivity of colostrum milkings in the days following calving.

Design

In vitro bioactivity used AGS, RIE-1 and Caco-2 cell proliferation (Alamar blue) and migration (wounded monolayers) assays. Changes in colostrum bioactivity determined following addition of TGFβ-neutralising antibody, EGFR blocker (Typhostin) and after heating

SUBMITTER: Playford RJ 

PROVIDER: S-EPMC7299325 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature