Dataset Information

New Method for Extracting and Purifying Dihydromyricetin from Ampelopsis grossedentata.

ABSTRACT: Dihydromyricetin (DMY) is a kind of flavone. It has a variety of physiological effects, and its content in Ampelopsis grossedentata is as high as 35%. There are two shortcomings in the traditional batch extraction process commonly used in a laboratory: long extraction time and low extraction rate. In this study, a new chelating extraction method was proposed, that is, Zn²⁺ was introduced into the extraction and purification process to chelate with DMY, and the yield and purity were taken as evaluation indices for a comparative study with the traditional batch extraction method. In addition, ¹H NMR, single-crystal X-ray diffraction, IR, and UV were used to analyze the product structure; thermogravimetry and differential thermal analysis was utilized to examine the thermal stability of DMY. The results were shown as follows. Compared with the batch extraction method, the chelation extraction method could effectively avoid the oxidation of DMY by air during the extraction and purification process, and the yield of the DMY also increased. Furthermore, this method was time-saving. Through investigating the extraction process and characterizing the structure and thermal stability of DMY, the chelating extraction method could be considered to provide a reference for commercial applications of DMY.

SUBMITTER: Hu H

PROVIDER: S-EPMC7301542 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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New Method for Extracting and Purifying Dihydromyricetin from Ampelopsis grossedentata.

Hu Hongchao H Luo Fan F Wang Mingjie M Fu Zhihuan Z Shu Xugang X

ACS omega 20200602 23

Dihydromyricetin (DMY) is a kind of flavone. It has a variety of physiological effects, and its content in Ampelopsis grossedentata is as high as 35%. There are two shortcomings in the traditional batch extraction process commonly used in a laboratory: long extraction time and low extraction rate. In this study, a new chelating extraction method was proposed, that is, Zn2+ was introduced into the extraction and purification process to chelate with DMY, and the yield and purity ...[more]

PMID: 32566862

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Project description:Oxidative stress is recognized as a significant contributor to the development and progression of inflammation and disruptions in the balance of gut microflora, commonly referred to as intestinal dysbiosis. It is crucial that safe and effective antioxidant and anti-inflammatory agents are identified to address these conditions. Ampelopsis grossedentata, a natural plant abundant in flavonoids and primarily found in southern China, has demonstrated potent antioxidant properties. However, the extent to which flavonoids in A. grossedentata impact intestinal inflammation and alter the composition of the gut microbiome remains to be fully understood. The purpose of this study was to explore the potential benefits of using A. grossedentata as an antioxidant and anti-inflammatory agent in the context of intestinal inflammation, both in vitro and in vivo. We first conducted an initial comparison of the effects of dihydromyricetin (DMY), an alcohol extract of A. grossedentata (AEA, 82% total flavonoids), and a water extract of A. grossedentata (WEA, 57% total flavonoids) on the cell viability and intestinal barrier integrity of porcine epithelial cells IPEC-J2. Although the total flavonoid content is much lower in WEA than in AEA, the results show that they have similar effects. Subsequently, the antioxidant properties of WEA were compared with those of commonly utilized antioxidants in vitro. Lastly, the antioxidant and anti-inflammatory properties of WEA, as well as its impacts on gut microbiota, were evaluated in animal models, including mice and Drosophila. In summary, the results of our study indicate that WEA, due to its antioxidant properties, exhibits a protective effect on the intestinal barrier function in porcine epithelial cell line IPEC-J2. Additionally, WEA demonstrates a positive correlation with DPPH, ABTS radical scavenging rate, FRAP, and reducing power under in vitro settings. Furthermore, WEA was shown to effectively alleviate oxidative stress in animal models by reducing the levels of pro-inflammatory cytokines and increasing the antioxidant enzyme activity in the liver, as well as by activating the Nrf2 signaling pathway in the duodenum. Additionally, WEA was able to regulate gut microbiota, promoting the growth of beneficial bacteria and inhibiting harmful microbes, as well as extending the lifespan of Drosophila. Overall, these findings suggest that WEA may serve as a valuable dietary supplement for addressing oxidative stress and inflammation through its anti-inflammatory and prebiotic effects, which are conferred via the Nrf2/Keap1 pathway.

Dataset Information

New Method for Extracting and Purifying Dihydromyricetin from Ampelopsis grossedentata.

Publications

New Method for Extracting and Purifying Dihydromyricetin from <i>Ampelopsis grossedentata</i>.

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