Enhanced sensitivity of VEGF detection using catalase-mediated chemiluminescence immunoassay based on CdTe QD/H₂O₂ system.

ABSTRACT:

Background

Since vascular endothelial growth factor (VEGF) is a significant regulator of cancer angiogenesis, it is essential to develop a technology for its sensitive detection. Herein, we sensitized a chemiluminescence (CL) immunoassay through the combination of H₂O₂-sensitive TGA-CdTe quantum dot (QD) as signal transduction, dextran as a cross-linker to prepare enzyme-labeled antigen and the ultrahigh bioactivity of catalase (CAT) as reporter enzyme.

Results

Under the optimized experimental conditions, the chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) method can detect VEGF in the excellent linear range of 2-35,000 pg mL^-1, with a detection limit (S/N = 3) of 0.5 pg mL^-1 which was approximately ten times lower t

SUBMITTER: Ghavamipour F 

PROVIDER: S-EPMC7302009 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature