The tumour microenvironment of the upper and lower gastrointestinal tract differentially influences dendritic cell maturation.

ABSTRACT:

Background

Only 10-30% of oesophageal and rectal adenocarcinoma patients treated with neoadjuvant chemoradiotherapy have a complete pathological response. Inflammatory and angiogenic mediators in the tumour microenvironment (TME) may enable evasion of anti-tumour immune responses.

Methods

The TME influence on infiltrating dendritic cells (DCs) was modelled by treating immature monocyte-derived DCs with Tumour Conditioned Media (TCM) from distinct gastrointestinal sites, prior to LPS-induced maturation.

Results

Cell line conditioned media from gastrointestinal cell lines inhibited LPS-induced DC markers and TNF-α secretion. TCM generated from human tumour biopsies from oesophageal, rectal and colonic adenocarcinoma induced different effects on LPS-induced DC markers -

SUBMITTER: Morrissey ME 

PROVIDER: S-EPMC7302160 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature