Dataset Information

Early upregulation of AR and steroidogenesis enzyme expression after 3 months of androgen-deprivation therapy.

ABSTRACT:

Background

Androgen deprivation therapy (ADT) is a standard treatment for advanced prostate cancer (PCa). However, PCa recurrence and progression rates during ADT are high. Until now, there has been no evidence regarding when progression begins. This study evaluated the gene expression of intraprostatic androgen receptor (AR) and steroidogenic enzymes in the early stages of ADT.

Methods

Prostate tissue samples were taken from PCa patients with urinary retention who received ADT (ADT-PCa; n?=?10) and were further subgrouped into ADT ?12?months (n?=?4) and ADT >?12?months (n?=?6). The ADT-PCa tissues were then compared with BPH (n?=?12) and primary (no treatment) PCa tissues (n?=?16). mRNA for gene expression analysis of AR and steroidogenic enzymes was extracted from formalin-fixed paraffin embedded (FFPE) tissues and analyzed by real-time PCR. Protein expression was evaluated by immunohistochemistry with specific antibodies.

Results

AR gene expression was higher in the ADT-PCa group than in the BPH or primary PCa group. Both the ADT ?12 and?>?12?months subgroups had significantly higher relative gene expression levels of AR (p??12?months subgroup compared with the PCa group (100%; p?ConclusionAR and steroidogenic enzymes are upregulated in ADT-PCa patients as early as 3?months, without PSA elevation. Steroidogenic enzymes, particularly SRD5A3, were also upregulated before PSA rose.

SUBMITTER: Hamid ARAH

PROVIDER: S-EPMC7304221 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Publications

Early upregulation of AR and steroidogenesis enzyme expression after 3 months of androgen-deprivation therapy.

Hamid Agus Rizal A H ARAH Kusuma Putra Harun W HW Sari Ningrum Paramita NP Diana Putri P Sesari Saras Serani SS Novita Eka E Gultom Fajar Lamhot FL Saraswati Meilania M Tanurahardja Budiana B Asmarinah Umbas Rainy R Mochtar Chaidir A CA

BMC urology 20200619 1

<h4>Background</h4>Androgen deprivation therapy (ADT) is a standard treatment for advanced prostate cancer (PCa). However, PCa recurrence and progression rates during ADT are high. Until now, there has been no evidence regarding when progression begins. This study evaluated the gene expression of intraprostatic androgen receptor (AR) and steroidogenic enzymes in the early stages of ADT.<h4>Methods</h4>Prostate tissue samples were taken from PCa patients with urinary retention who received ADT (A ...[more]

PMID: 32560654

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Dataset Information

Early upregulation of AR and steroidogenesis enzyme expression after 3 months of androgen-deprivation therapy.

Background

Methods

Results

Publications

Early upregulation of AR and steroidogenesis enzyme expression after 3 months of androgen-deprivation therapy.

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